bay k 8644 bayk alomone labs b 351 50 mm in dmso  (Alomone Labs)

Journal: mSphere

Article Title: The pan-variant potential of light: 425 nm light inactivates SARS-CoV-2 variants of concern and non-cytotoxic doses reduce viral titers in human airway epithelial cells

doi: 10.1128/msphere.00230-25

Figure Lengend Snippet: 425 nm light reduces SARS-CoV-2 Beta, Delta, and Omicron titers at non-cytotoxic doses in ALI HAE. Well-differentiated human large airway epithelial cells ( A ) were illuminated twice daily (BID) with 16 or 32 J/cm 2 of 425 nm light ( B ). At 72 hpi (six total doses), cell viability was determined via 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay ( C ). Data presented are the mean viability ± SEM relative to 0 J/cm 2 ( n = 6). ( D ) Plates coated with synthetic human ACE-2 were illuminated with 425 nm light and inoculated with recombinant spike trimers derived from WA1 and Omicron. Data presented are the mean percent binding to ACE-2 relative to 0 J/cm 2 ± SEM ( n = 5). ( E, F ) ALI HAE cells were infected with SARS-CoV-2 Beta, Delta, or Omicron variants, treated, and illuminated twice daily for 3 days with 32 J/cm 2 of 425 nm light starting at 3 hpi (MOI 0.1 [ E ]) or 24 hpi (MOI 0.001 [ F ]). Apical rinses were collected daily and enumerated via plaque assay. Data presented are the mean viral titer (PFU/mL) ± SEM ( n = 5–6). Statistical significance was determined with the Mann-Whitney rank-sum test and is indicated by * ( P < 0.05) and ** ( P < 0.01). For panels C and D, statistical significance was determined relative to the 0 J/cm 2 group. For panels E and F, statistical significance was determined relative to the 0 J/cm 2 group at the corresponding timepoint.

Article Snippet: Plates were blocked with 200 μL blocking buffer (5% FBS in PBST) and incubated at 37°C/5% CO 2 for 1 h. His-tagged recombinant WA1 S1 (Sino Biological #40591-V08H), Omicron B.1.1.529 S1 (Sino Biological #40591-V08H41), MERS-CoV S1 (Sino Biological #40069-V08H), SARS-CoV-1 S1+S2 (Sino Biological #40634-V08B), NL63 S1+S2 (Sino Biological #40604-V08B), MERS-CoV S1+S2 (Sino Biological #40069-V08B), WA1 spike trimer (Sino Biological #40589-V08H4), Alpha spike trimer (Sino Biological #40589-V08H12), Beta spike trimer (Sino Biological #40589-V08H13), Gamma spike trimer (Sino Biological #40589-V08H23), Delta spike trimer (Sino Biological #40589-V08H10), Lambda spike trimer (Sino Biological #40589-V08H24), Omicron B.1.1.529 spike trimer (Sino Biological #40589-V08H26), Omicron BA.2 spike trimer (Sino Biological #40589-V08H28), Omicron XBB.1.5 spike trimer (40589-V08H45), and Omicron JN.1 spike trimer (Sino Biological #40589-V08H59) were diluted in viral diluent.

Techniques: MTT Assay, Recombinant, Derivative Assay, Binding Assay, Infection, Plaque Assay, MANN-WHITNEY

Journal: mSphere

Article Title: The pan-variant potential of light: 425 nm light inactivates SARS-CoV-2 variants of concern and non-cytotoxic doses reduce viral titers in human airway epithelial cells

doi: 10.1128/msphere.00230-25

Figure Lengend Snippet: The RD-X19 reduces spike binding to ACE-2, inactivates SARS-CoV-2, and reduces viral titers at non-cytotoxic doses. ( A ) Recombinant spike trimers derived from WA1 and Omicron JN.1 were assessed for receptor binding following illumination with the active RD-X19 or sham. Data presented are the mean percent binding to ACE-2 relative to unilluminated ± SEM ( n = 4). ( B ) SARS-CoV-2 Beta and Gamma were diluted in cell culture media and illuminated with the active RD-X19 or sham. Data presented are the mean viral titer (PFU/mL) ± SEM ( n = 8). ( C ) ALI HAE cell cultures were illuminated twice daily (BID) with the Active RD-X19 or Sham for 3 days (six total doses). Cell viability was determined via MTT assay. Data presented are the mean viability ± SEM relative to unilluminated ( n = 4). ( D ) ALI HAE cell cultures were infected with SARS-CoV-2 WA1 illuminated twice daily for 3 days with the active RD-X19 or sham starting at 3 or 24 hpi. Apical rinses were collected daily and enumerated via plaque assay. Data presented are the mean viral titer (PFU/mL) ± SEM ( n = 10). Statistical significance was determined with the Mann-Whitney rank-sum test and is indicated by * ( P < 0.05), ** ( P < 0.01), and *** ( P < 0.001). For panel A, statistical significance was determined relative to the 0 J/cm 2 group. For panel B, statistical significance was determined relative to the inoculum. For panel D, statistical significance was determined relative to the unilluminated group at the corresponding timepoint.

Article Snippet: Plates were blocked with 200 μL blocking buffer (5% FBS in PBST) and incubated at 37°C/5% CO 2 for 1 h. His-tagged recombinant WA1 S1 (Sino Biological #40591-V08H), Omicron B.1.1.529 S1 (Sino Biological #40591-V08H41), MERS-CoV S1 (Sino Biological #40069-V08H), SARS-CoV-1 S1+S2 (Sino Biological #40634-V08B), NL63 S1+S2 (Sino Biological #40604-V08B), MERS-CoV S1+S2 (Sino Biological #40069-V08B), WA1 spike trimer (Sino Biological #40589-V08H4), Alpha spike trimer (Sino Biological #40589-V08H12), Beta spike trimer (Sino Biological #40589-V08H13), Gamma spike trimer (Sino Biological #40589-V08H23), Delta spike trimer (Sino Biological #40589-V08H10), Lambda spike trimer (Sino Biological #40589-V08H24), Omicron B.1.1.529 spike trimer (Sino Biological #40589-V08H26), Omicron BA.2 spike trimer (Sino Biological #40589-V08H28), Omicron XBB.1.5 spike trimer (40589-V08H45), and Omicron JN.1 spike trimer (Sino Biological #40589-V08H59) were diluted in viral diluent.

Techniques: Binding Assay, Recombinant, Derivative Assay, Cell Culture, MTT Assay, Infection, Plaque Assay, MANN-WHITNEY